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为探讨共刺激分子B7-H4在牛Ⅱ型胶原(CⅡ)诱导的类风湿关节炎(CIA)模型小鼠免疫器官、关节及RA患者滑膜组织内的表达,使用牛Ⅱ型胶原免疫DBA-1/j小鼠,建立小鼠类风湿关节炎模型。免疫组化检测B7-H4的表达变化,结果证实小鼠的胸腺、脾脏及淋巴结中有大量的B7-H4阳性细胞,免疫荧光分析结果表明这些B7-H4+细胞主要为CD31+内皮细胞。统计分析表明,CIA小鼠体内免疫器官中B7-H4表达及分布相对于对照组小鼠没有显著提高,但是RA患者及CIA小鼠的滑膜组织内发现大量B7-H4阳性细胞。鉴于关节炎滑膜组织内有大量B7-H4阳性细胞,提示其有可能参与并调节了关节炎的病理进程。
To investigate the expression of costimulatory molecule B7-H4 in the synovial tissue of immune organs, joints and RA patients induced by C Ⅱ-induced rheumatoid arthritis (CIA) in mice, the expression of DBA- 1 / j mice to establish mouse rheumatoid arthritis model. B7-H4 expression was detected by immunohistochemistry. The results showed that a large number of B7-H4 positive cells were found in the thymus, spleen and lymph nodes of mice. Immunofluorescence analysis showed that these B7-H4 + cells were predominantly CD31 + endothelial cells. Statistical analysis showed that B7-H4 expression and distribution in immune organs of CIA mice were not significantly increased compared with control mice, but a large number of B7-H4 positive cells were found in synovial tissues of RA patients and CIA mice. Arthritis synovial tissue in view of a large number of B7-H4 positive cells, suggesting that it may participate in and regulate the pathological process of arthritis.