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根据前期实验获得的大豆Gm BIN2基因登录号,从大豆中克隆Gm BIN2基因的全长CDS序列,得到大豆Gm BIN2基因。对大豆再生相关基因Gm BIN2的启动子序列、氨基酸序列、编码的蛋白质结构、亲疏水性以及同源进化树进行分析,结果表明,大豆再生相关基因Gm BIN2编码区c DNA长度为1 125 bp,编码374个氨基酸,Gm BIN2编码的蛋白为亲水性蛋白;分析其蛋白功能结构域发现,Gm BIN2蛋白具有丝氨酸/苏氨酸激酶催化域,为PKc-like超家族成员;构建系统进化树发现其与野生大豆亲缘较近。本研究的实验结果有利于更加深入的研究Gm BIN2基因在大豆再生过程中的关键作用,为提高大豆再生效率提供依据。
According to the Gm BIN2 gene accession number obtained from previous experiments, the full-length CDS sequence of Gm BIN2 gene was cloned from soybean to obtain soybean Gm BIN2 gene. Analysis of the promoter sequence, amino acid sequence, encoded protein structure, hydrophilicity and hydrophobicity, and homologous phylogenetic tree of Gm BIN2 related to soybean regeneration showed that the length of c DNA of Gm BIN2 coding region of soybean regeneration gene was 1 125 bp, encoding 374 amino acids. The protein encoded by Gm BIN2 was a hydrophilic protein. The protein functional domain of Gm BIN2 protein was found to have serine / threonine kinase catalytic domain, which is a member of PKc-like superfamily. The phylogenetic tree of Gm BIN2 protein was found Close proximity to wild soy. The experimental results of this study are conducive to a more in-depth study of the key role of Gm BIN2 gene in soybean regeneration process to provide a basis for improving soybean regeneration efficiency.