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血液和血液制品是丙型肝炎的主要传播方式之一，供血者健康检查标准规定凡ＥＬＩＳＡ检测抗ＨＣＶ抗体阳性者均不得献血。但ＥＬＩＳＡ检测存在着漏检及假阳性问题，并且只有ＨＣＶ－ＲＮＡ阳性血清才具有感染性。因此，我们对某血站送检的１００例抗ＨＣＶ阳性献血员的血样进行了ＨＣＶ－ＲＮＡ检测，结果表明，在ＨＣＶ－ＲＮＡ半套式ＲＴ－ＰＣＲ中，传统的异硫氰酸胍／氯仿／异戌醇方法同磁珠法抽提病毒ＲＮＡ，结果两种方法同样可以得到理想的２４０ｂｐ扩增带。用传统方法进行实验，１００例血标本中３例ＨＣＶ－ＲＮＡ阴性，其它均阳性，说明我国制定的献血员标准规定绝大多数情况下是适宜的，如果结合ＲＴ－ＰＣＲ检测ＨＣＶ－ＲＮＡ，结果会更加理想。这为库血ＨＣＶ检测与献血员ＨＣＶ感染检测提供了一种可靠的检测方法，对防止输血后肝炎的发生、净化献血员有一定的指导意义
Blood and blood products are one of the main modes of transmission of hepatitis C. Health examination standards for blood donors are not allowed to donate blood if they are tested positive for anti-HCV antibodies by ELISA. However, there is a problem of undetected and false positive ELISA tests, and only HCV-RNA positive sera are infectious. Therefore, we tested the blood samples of 100 anti-HCV positive blood donors from a blood test station for HCV-RNA. The results showed that in the HCV-RNA semi-sheathed RT-PCR, the traditional guanidine isothiocyanate / chloroform / Iso-amyl alcohol method with magnetic beads extraction of viral RNA, the results of the two methods can also be the ideal 240bp amplification band. In the traditional method, three of the 100 blood samples were negative for HCV-RNA, and the others were all positive. This shows that the standard of blood donors in our country is suitable for the vast majority of cases. If HCV-RNA is detected by RT-PCR, the result Will be more ideal. This provides a reliable method for the detection of HCV in blood serum and the detection of HCV infection in blood donors and has certain guiding significance for preventing the occurrence of hepatitis after blood transfusion and purifying blood donors