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目的:建立血浆中阿呋唑嗪的反相高效液相荧光检测方法。方法:采用Shimadzu LC-6高效液相色谱仪,ShimadzuC_(18)柱(150mm×4.6 mm),0.02 mol·L~(-1)磷酸盐缓冲液(pH2.5)-乙腈(40:60)为流动相,激发波长374nm,发射波长378nm,流速:1mL·min~(-1)。结果:血浆中阿呋唑嗪能得到较好的分离,无明显的干扰峰。本方法专属性好,可信度高。最低检测浓度为0.78ng·mL~(-1),最低检测限是39pg,线性范围0.78～50 ng·mL~(-1),标准曲线:Y=8648.7X+767.8(r=0.999,P<0.05),回收率高于70%,日间、日内RSD均在10％以下。结论:符合生物样品的分析要求。
Objective: To establish a method for the determination of alfuzosin in plasma by reversed-phase high performance liquid chromatography. METHODS: Shimadzu LC-6 high performance liquid chromatography (HPLC) was used with Shimadzu C 18 column (150 mm × 4.6 mm) and 0.02 mol·L -1 phosphate buffer (pH 2.5) As mobile phase, excitation wavelength 374nm, emission wavelength 378nm, flow rate: 1mL · min ~ (-1). Results: There was a good separation of alfuzosin in plasma with no obvious interference peak. The method is good in specificity and high in credibility. The lowest detection limit was 0.78ng · mL -1, the lowest detection limit was 39pg, the linear range was 0.78 ~ 50 ng · mL -1, the standard curve was Y = 8648.7X + 767.8 (r = 0.999, P < 0.05), the recovery rate is higher than 70%, day and day RSD are below 10%. Conclusion: In line with the biological sample analysis requirements.