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为了明确芒果炭疽病病菌(Colletotrichum gloeosporioides)漆酶与该菌侵染相关致病因子之间的关系,本文以愈创木酚为底物筛选获得的高产漆酶菌株A2为材料,测定了漆酶粗提液对芒果炭疽病病菌致病力的影响,并通过半定量RT-PCR法,分析了在不同侵染时段漆酶基因lac1和其他酶基因的表达。结果表明,漆酶粗提液单独不能致病,但能促进该病原菌在寄主中的扩展;在侵染过程中,lac1表达与黑色素合成酶(thd和scd)基因表达相关性更高,与细胞壁降解酶(ecg和pel)基因表达也有一定相关性,但不及前者高。芒果胶孢炭疽菌分泌的漆酶有助于该菌在侵染芒果过程中的扩展,且对侵染相关致病因子黑色素合成酶和细胞壁降解酶基因的表达有一定影响。初步推测,漆酶lac1除了能通过降解芒果组织中的酚类物质、促进病原菌的扩展之外,也可以通过干扰与致病力相关的黑色素合成和细胞壁降解酶的产生来影响芒果胶孢炭疽菌致病力。
In order to clarify the relationship between the laccase of Colletotrichum gloeosporioides and the virulence factors associated with the infection of this bacterium, high-yield laccase strain A2 screened with guaulol as substrate was used as material to determine the laccase Crude extract on the pathogenicity of the anthracnose pathogen of mango and semi-quantitative RT-PCR method was used to analyze the expression of lac1 and other enzymes at different infection stages. The results showed that the laccase crude extract alone could not cause disease, but could promote the expansion of the pathogen in the host. In the process of infection, the expression of lac1 was correlated with the gene expression of melanin synthase (thd and scd) Degrading enzyme (ecg and pel) gene expression is also a certain correlation, but not as high as the former. The laccase secreted by Colletotrichum gloeosporioides could promote the expansion of the bacteria in the process of mango infection, and also affect the expression of genes related to melanin synthase and cell wall degrading enzymes. It was preliminarily speculated that Lacc lac1 could not only promote the expansion of pathogenic bacteria by degrading the phenolic compounds in mango tissue, but also interfere with the pathogenicity of melanin synthesis and cell wall degrading enzymes Pathogenicity.