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目的:探讨姜黄素对3T3-L1前脂肪细胞增殖、分化的影响及其机制。方法:以XTT法检测3T3-Ll前脂肪细胞的增殖;油红O染色并通过比色定量分析检测3T3-L1前脂肪细胞分化过程中胞浆脂质的堆积,实时定量荧光PC(real-time-PCR)检测脂肪细胞增殖、分化相关基因内脂素、抵抗素、脂联素mRNA的表达。结果:姜黄素组A值显著高于空白对照组并随着剂量增加而增大,且具有显著性差异(P<0.05);姜黄素作用细胞48 h时,促进或抑制细胞增殖的作用最强,40μmol/L以上浓度的姜黄素出现细胞毒性作用,大部分细胞成片脱落死亡;72 h时,15~35μmol/L姜黄素组细胞的生长率有所增加,而40μmol/L以下姜黄素处理组细胞生长率仍维持在较低水平;脂肪细胞油红O染色分光光度法结果显示2.5,5,10,15和20μmol/L姜黄素剂量组的细胞吸光度值与空白对照组相比有显著性差异(P<0.05);姜黄素组、吡格列酮组的内脂素、抵抗素mRNA表达均较对照组显著降低,而脂联素较对照组明显升高,且具有显著性差异(P<0.05)。结论:姜黄素能够促进前脂肪细胞分化,低浓度时促进其增殖,高浓度时则抑制其增殖,降低内脂素、抵抗素mRNA表达,促进脂联素mRNA的表达。
Objective: To investigate the effect and mechanism of curcumin on proliferation and differentiation of 3T3-L1 preadipocytes. Methods: The proliferation of 3T3-L1 preadipocytes was detected by XTT method. The accumulation of cytoplasmic lipids in 3T3-L1 preadipocytes was detected by oil red O staining and colorimetric quantitative analysis. The real-time -PCR) was used to detect the expression of adiponectin, resistin and adiponectin mRNA in adipocyte proliferation and differentiation-related genes. Results: The A value of curcumin group was significantly higher than that of blank control group and increased with the increase of dose (P <0.05). Curcumin promoted the proliferation of cells at 48 h Curcumin at a concentration above 40μmol / L showed cytotoxicity, and most of the cells died of apoptosis. At 72h, the growth rate of curcumin at 15 ~ 35μmol / L increased, but curcumin at 40μmol / L The cell growth rate of the group remained at a low level; the results of oil red O staining of adipocytes showed that the cell absorbance values of 2.5, 5, 10, 15 and 20 μmol / L curcumin dose groups were significant compared with the blank control group (P <0.05). The expressions of visfatin and resistin mRNA in curcumin group and pioglitazone group were significantly lower than those in control group (P <0.05), while the levels of adiponectin in control group were significantly higher than those in control group (P <0.05) . CONCLUSION: Curcumin can promote the differentiation of preadipocytes, promote its proliferation when the concentration is low, inhibit its proliferation, decrease the expression of visfatin and resistin, and promote the expression of adiponectin mRNA.