采用Ｃ１８色谱柱和紫外检测器，对病虫清可湿性粉剂中的三种活性成分噻嗪酮、杀虫单和井冈霉素的含量进行反相高效液相色谱测定，以外标法面积定量。噻嗪酮、杀虫单和井冈霉素的变异系数分别为０５１％、０６２％、１０８％，回收率为９９４５％～１００６５％、９５４４％～９９２３％、９７２５％～９９３６％，线性相关系数为０９９８２，０９９８５，０９９８３。方法用于实样测定，结果令人满意。 The C18 column and UV detector were used to determine the contents of three active ingredients buprofezin, pemmatism and validamycin in pest and disease WP by reversed-phase high performance liquid chromatography and quantified by external standard method. The coefficients of variation for buprofezin, pendimethalin and validamycin were 051%, 062%, 108%, respectively. The recoveries were 9945% -10065%, 9544% ~ 99  23%, 9725% ~ 9936%, linear correlation coefficient of 0  9982,0  9985,0  9983. The method was used for real sample determination with satisfactory results.