本文建立了中草药黄芩中黄芩甙、青黛中靛蓝、牡丹皮中丹皮酚、荜茇中胡椒碱的二阶导数差示脉冲极谱的定量分析方法。黄芩、靛蓝、丹皮酚和胡椒碱的化学结构中都含有ＣＯ，在酸性条件下，于滴汞电极上均可发生还原反应生成ＣＯ，并分别于１５５０Ｖ，１３００Ｖ，１６３０Ｖ，０８６０Ｖ（ｖｓＡｇ／ＡｇＣｌ）处出现良好的二阶导数差示脉冲极谱峰。黄芩、靛蓝、丹皮酚和胡椒碱分别在４５×１０５～２７×１０４ｍｏｌ·Ｌ１，３８×１０５～２５×１０４ｍｏｌ·Ｌ１，１０～６０×１０４ｍｏｌ·Ｌ１和７０×１０５～２５×１０４ｍｏｌ·Ｌ１范围内，浓度与其二阶导数差示脉冲极谱峰幅值呈极为显著的线性关系（Ｐ〈００１），检测限分别为９０×１０８ｍｏｌ·Ｌ１，８４×１０９ｍｏｌ·Ｌ１，９２×１０９ｍｏｌ·Ｌ１和８７×１０９ｍｏｌ·Ｌ１。方法简便、快速、灵敏，结果准确。 In this paper, the second derivative differential pulse polarographic analysis method was established for the Chinese herb Astragalus membranaceus, Amaranthus indigo, Paeonia suffruticosa and piperine in peony bark. The chemical structures of Astragalus, Indigo, paeonol and piperine contain CO. Under acidic conditions, reduction reactions can occur at the mercury drop electrode to generate CO, and they can be separated from 1550V, 1300V, respectively. Good second-order derivative pulse polar peaks appear at 1630V, 0860V (vsAg/AgCl). Astragalus membranalis, indigo, paeonol, and piperine were found at 45×10−5～27×104mol·L1,38×10−5~25×104mol·L. In the range of 1,10～60×104mol·L1 and 70×105~25×104mol·L1, the concentration and its second derivative are different. The peak amplitude showed an extremely significant linear relationship (P<001), and the detection limits were 90×10−8mol·L1,84×109mol·L1,92×10 respectively. 9mol·L1 and 87×109mol·L1. The method is simple, rapid, sensitive and accurate.