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目的 探讨原发性前列腺癌及高级别前列腺上皮内肿瘤 (PIN ) 2 2条常染色体等位基因杂合性缺失及其意义。方法采用显微切割技术提取前列腺癌及高级别前列腺上皮内肿瘤各 10例的DNA ,然后采用PCR及微卫星多态性技术 ,对 2 2条常染色体上的 382个微卫星标志位点进行高密度allelotype分析。 结果 10例原发性前列腺癌在染色体 1p、1q、2 p、2 q、6q、8p、8q、10q、11q、13q、16p、17p等部位存在高频杂合性缺失 (LOH ) ,前列腺上皮内肿瘤则在染色体 2 p、2q、6q、8p、10q、1q等区易检测到LOH。结论前列腺癌患者 2 q14、8p12～ 2 1、10 q2 3～ 2 4、13q14等区域存在高频的LOH ,位于这些区域的抑癌基因在前列腺癌发生、发展过程中起着重要作用
Objective To investigate the loss of heterozygosity of 22 autosomal alleles in primary prostate cancer and high-grade prostatic intraepithelial neoplasia (PIN) and its significance. Methods DNA was extracted from 10 cases of prostate cancer and 10 cases of high-grade prostate intraepithelial neoplasia by microdissection. Then, 382 microsatellite loci on 22 autosomes were amplified by PCR and microsatellite polymorphism Density allelotype analysis. Results The 10 cases of primary prostate cancer had high frequency heterozygous deletion (LOH) on the chromosomes 1p, 1q, 2p, 2q, 6q, 8p, 8q, 10q, 11q, 13q, 16p and 17p. The tumor in the chromosome 2 p, 2q, 6q, 8p, 10q, 1q and other areas easily detect LOH. Conclusions High frequency LOH exists in the regions of 2q14, 8p12 ~ 21, 10q2 ~ 3, 14q, 13q14 in prostate cancer patients. The tumor suppressor genes located in these regions play an important role in the development and progression of prostate cancer