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目的 探讨先天性心脏病与宫内肠道病毒感染的关系。方法 用聚合酶链反应技术及核酸杂交方法检测先天性心脏病患儿心肌组织及血清中肠道病毒RNA,同时检测患儿血清中柯萨奇B病毒特异IgM、IgG抗体,取非心脏病原因死亡的新生儿心肌标本及非心脏病人的血清作为对照。结果 先天性心脏病患儿心肌标本中肠道病毒RNA检测阳性率明显高于非心脏病组,而血清柯萨奇B特异IgM、IgG抗体检测阳性率与非心脏病组差异无显著性。结论 先天性心脏病可能与宫内肠道病毒感染有关,且生后病毒仍持续存在于心肌组织中。
Objective To investigate the relationship between congenital heart disease and intrauterine enterovirus infection. Methods Polymerase chain reaction (PCR) and nucleic acid hybridization were used to detect the viral RNA in myocardium and serum of patients with congenital heart disease, and to detect the Coxsackie B virus-specific IgM and IgG in serum of children with congenital heart disease. Non-heart disease Death of neonatal myocardial specimens and non-cardiac patients serum as a control. Results The positive rate of enterovirus RNA in myocardial samples of children with congenital heart disease was significantly higher than that of non-heart disease patients. However, the positive rate of coxsackie B-specific IgM and IgG antibody was not significantly different from non-heart disease group. Conclusions Congenital heart disease may be related to intrauterine enterovirus infection, and the virus persists in myocardium after birth.