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目的对山东省1997~2004年急性弛缓性麻痹(AFP)病例中疫苗变异脊髓灰质炎(脊灰)病毒的分子生物学性状进行分析,以提高对疫苗衍生脊灰病毒(VDPV)的认识,从而增加AFP病例监测系统检出VDPV的灵敏度。方法分离的脊灰病毒血清定型采用中和试验的方法,型内鉴定采用酶联免疫吸附试验(ELISA)和聚合酶链反应-限制性酶切片段长度多态性分析(PCR-RFLP)两种方法,疫苗变异脊灰病毒分子生物学性状分析采用VP1区序列测定的方法。结果山东省1997~2004年未分离到脊灰野病毒,分离到的脊灰疫苗病毒以Ⅱ型为主(173株),接近Ⅰ型和Ⅲ型之和。其中疫苗变异脊灰病毒10株,仍以Ⅱ型为主(8株),且有1株(CHN1025)VP1区变异率达到1%,为VDPV,但该毒株无2代病例发生。结论在山东省连续多年的口服脊灰疫苗免疫已经阻断了脊灰野病毒在人群中的传播,但从AFP病例中曾分离到1株VDPV,结合流行病学分析发现,局部地区仍存在免疫空白,因此存在着VDPV以及发生循环的VDPV潜在的可能,应引起相关部门的关注。
Objective To analyze the molecular biological characteristics of the vaccine poliovirus (poliovirus) in acute flaccid paralysis (AFP) cases in Shandong Province from 1997 to 2004 so as to improve the understanding of vaccine-derived poliovirus (VDPV) Increase the sensitivity of the AFP case monitoring system to detect VDPV. The method of neutralization was used to determine the serotypes of poliovirus isolated in this study. ELISA and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) Methods, vaccine variation Poliovirus molecular biology traits using VP1 region sequence determination method. Results There was no wild poliovirus isolated from Shandong Province from 1997 to 2004. The poliovirus isolated from Shandong Province was mainly type Ⅱ (173 strains), close to the sum of type Ⅰ and type Ⅲ. Among them, 10 strains of poliovirus were still type Ⅱ (8 strains), and 1 strain (CHN1025) had VP1 region mutation rate of 1%, which was VDPV. However, there was no case of generation 2 in this strain. Conclusion The oral poliovirus vaccine in Shandong Province for many years has blocked the transmission of poliovirus in the population. However, one VDPV was isolated from the AFP cases. Combined with the epidemiological analysis, it was found that the immune blank still existed in some areas Therefore, the potential of VDPV and circulating VDPV exists and should arouse the concern of relevant departments.