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目的探讨髓系细胞触发受体1(TREM-1)在宫内感染新生大鼠中的表达及其诊断价值。方法 30只SD孕鼠随机分为正常对照组(A组,6只)和宫内感染模型组(B组,24只),腹腔注射脂多糖0.5mg/kg建立宫内感染模型,并分别于幼鼠出生后1、3、7、14d留取外周血及脑、肺组织。采用Western blot法和免疫荧光法检测脑组织和肺组织中TREM-1表达,ELISA法检测外周血TREM-1和TNF-α水平。结果 A组未见明显的TREM-1阳性染色细胞表达。与A组相比,B组新生大鼠脑组织、肺组织中TREM-1蛋白表达以及外周血中TREM-1、TNF-α水平在生后1d明显升高,3d达到峰值,14d逐渐降低。TREM-1和TNF-α表达水平呈正相关(r=0.794,P<0.01)。结论宫内感染后,新生大鼠脑、肺组织及外周血中TREM-1表达明显升高,且与TNF-α水平密切相关,有望成为早期诊断脓毒症的有用指标之一。
Objective To investigate the expression of myeloid cell trigger receptor 1 (TREM-1) in neonatal rats with intrauterine infection and its diagnostic value. Methods Thirty pregnant SD pregnant rats were randomly divided into normal control group (group A, n = 6) and intrauterine infection group (group B, n = 24). Intraperitoneal injection of lipopolysaccharide 0.5 mg / kg was used to establish intrauterine infection model. At 1, 3, 7 and 14 days after birth, peripheral blood, brain and lung tissues were collected. The expression of TREM-1 in brain and lung tissues was detected by Western blot and immunofluorescence. The levels of TREM-1 and TNF-α in peripheral blood were detected by ELISA. Results There was no obvious expression of TREM-1 positive cells in group A. Compared with group A, the expression of TREM-1 in peripheral blood and the level of TREM-1 and TNF-α in brain tissue and lung tissue of group B were significantly increased at 1 day after birth, peaked at 3d, and gradually decreased at 14 days. There was a positive correlation between the expression of TREM-1 and TNF-α (r = 0.794, P <0.01). Conclusion After intrauterine infection, the expression of TREM-1 in the brain, lung tissue and peripheral blood of neonatal rats is significantly increased, and closely related to the level of TNF-α, which is expected to be one of the useful indicators for the early diagnosis of sepsis.