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目的：研究ｐ５３及ｃｅｒｂＢ２蛋白在胆囊良恶性病变中的表达及意义，探讨它们在胆囊癌早期诊断和鉴别诊断中的潜在价值。方法：采用免疫组化ＳＰ法及微波抗原修复法检测２９例胆囊癌及２１例胆囊良性病变的ｐ５３蛋白的表达，同时仅用ＳＰ法检测ｃｅｒｂＢ２蛋白的表达。结果：ｐ５３蛋白在胆囊癌中表达的阳性率为５１７％，而在良性病变中无表达（Ｐ＜００５）；ｐ５３蛋白的表达在早晚期胆囊癌中无显著性差异（Ｐ＞００５）；仅３例胆囊癌ｃｅｒｂＢ２阳性表达，其中１例ｐ５３亦阳性。结论：ｐ５３蛋白检测可做为胆囊癌早期诊断及鉴别诊断的一个重要指标
Objective: To investigate the expression and significance of p53 and cerbB2 proteins in benign and malignant gallbladder lesions, and to explore their potential value in the early diagnosis and differential diagnosis of gallbladder cancer. Methods: The expression of p53 protein was detected in 29 cases of gallbladder carcinoma and 21 cases of benign gallbladder lesions by immunohistochemical SP method and microwave antigen retrieval method. The expression of cerbB2 protein was only detected by SP method. RESULTS: The positive rate of p53 protein in gallbladder carcinoma was 51.7%, but it was not expressed in benign lesions (P<0.05). The expression of p53 protein was not significantly different in early gallbladder carcinoma (P>0.05). 0 05); only 3 cases of gallbladder carcinoma c erbB 2 positive expression, 1 case of p53 also positive. Conclusion: The detection of p53 protein can be used as an important indicator for the early diagnosis and differential diagnosis of gallbladder carcinoma.