目的研究重组反义ｃｍｙｃ腺病毒对胃癌细胞的体内外生物学作用．方法采用ＬａｃＺ基因Ｘｇａｌ染色、ＭＴＴ，ＤＮＡ梯度降解试验、原位末端标记、流式细胞仪、ＰＣＲ分析、裸鼠致瘤性、裸鼠皮下移植瘤模型实验等方法，对反义ｃｍｙｃ重组腺病毒在人胃癌ＳＧＣ７９０１细胞系中的作用进行体内外研究．结果ＡｄＡＳｃｍｙｃ对ＳＧＣ７９０１细胞能产生明显的生长抑制作用，ＭＴＴ显示生长抑制率为４４１％．ＤＮＡ梯度降解试验、原位末端标记、流式细胞仪显示ＡｄＡＳｃｍｙｃ诱导了ＳＧＣ７９０１细胞凋亡．经ＡｄＡＳｃｍｙｃ处理的ＳＧＣ７９０１细胞裸鼠致瘤性消失．ＡｄＡＳｃｍｙｃ对裸鼠皮下移植瘤模型瘤内注射能有效降低肿瘤的生长速度，生长抑制率为６８９％．结论ＡｄＡＳｃｍｙｃ对胃癌细胞具有显著的体内外生长抑制及凋亡诱导作用 Objective To study the in vitro and in vivo biological effects of recombinant antisense cmyc adenovirus on gastric cancer cells. Methods LacZ gene X  gal staining, MTT, DNA gradient degradation assay, in situ end labeling, flow cytometry, PCR analysis, tumorigenicity in nude mice, nude mice subcutaneous xenograft model experiments, etc., antisense c  The role of myc recombinant adenovirus in human gastric cancer SGC7901 cell line was studied in vitro and in vivo. RESULTS Ad-AScmyc could significantly inhibit the growth of SGC7901 cells. MTT assay showed a growth inhibition rate of 44.1%. DNA gradient degradation assay, in situ end labeling and flow cytometry showed that Ad-AScmyc induced apoptosis in SGC7901 cells. The tumorigenicity of SGC7901 cells treated with Ad-AScmyc disappeared. Intratumoral injection of Ad-AScmyc in the subcutaneous xenograft model of nude mice can effectively reduce the growth rate of the tumor, and the growth inhibition rate is 689%. Conclusion Ad-AScmyc has significant in vivo and in vitro growth inhibition and apoptosis induction in gastric cancer cells.