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目的:研究外源性pten基因转染对人胃癌BGC823细胞PI3’K/AKT耐药通路的影响。方法:以无内源性pten表达的人胃癌BGC823细胞株转染PEAK8空载质粒和PEAK8-pten质粒,给予足叶乙苷和阿霉素诱导,RT-PCR方法分析目的基因的表达,免疫沉淀后Western-blot检测AKT活性。结果:RT-PCR显示转染pEAK8-pten质粒组有pten强表达。pten基因的导入对化疗药诱导的AKT活性有抑制作用。结论:pten基因转染能抑制人胃癌BGC823细胞体外生长,促进凋亡,并通过抑制AKT活性加强化疗药物的疗效。
Objective: To study the effect of exogenous pten gene transfection on the PI3’K/AKT drug resistance pathway in human gastric cancer BGC823 cells. METHODS: PEAK8 empty vector and PEAK8-pten plasmid were transfected into human gastric cancer BGC823 cell line without endogenous pten expression. Erythropoietin and doxorubicin were induced. Expression of target gene was analyzed by RT-PCR and immunoprecipitation was performed. Post-Western-blot detects AKT activity. Results: RT-PCR showed that pten was strongly expressed in the pEAK8-pten plasmid group. The introduction of pten gene has an inhibitory effect on AKT activity induced by chemotherapeutic agents. Conclusion: The transfection of pten gene can inhibit the growth of human gastric cancer BGC823 cells in vitro, promote apoptosis, and enhance the efficacy of chemotherapy drugs by inhibiting AKT activity.