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目的 通过观察重组肝再生增强因子（rALR）。苦参素和丹参对大鼠肝脏星形细胞（HSC）株IG12增殖及细胞外基质合成的影响,以研究其抗肝纤维化的疗效。方法 利用DNA重组、转导、诱导及纯化等一系列方法制备肝再生增强因子重组蛋白,利用胶原酶灌注及密度梯度离心法分离肝星形细胞,用有限稀释法建立大鼠HSC株IG12;于体外观察rALR、苦参素和丹参作用于IG12后IG12酸性磷酸酶活力及细胞外基质合成的改变。结果rALR、苦参素和丹参均可显著抑制IG12的增殖,并选择性地抑制其细胞外基质的合成。结论rALR、苦参素和丹参均具有抗肝纤维化的作用;IG12对于抗肝纤维化药物的筛选具有很大的价值。
Objective To observe the recombinant liver regeneration augmentation factor (rALR). The effects of oxymatrine and salvia miltiorrhiza on the proliferation and extracellular matrix synthesis of rat hepatic stellate cell (HSC) strain IG12 were studied in order to investigate its anti-hepatic fibrosis effect. Methods Recombinant protein of liver regeneration factor was prepared by a series of methods such as DNA recombination, transduction, induction and purification. The hepatic stellate cells were isolated by collagenase perfusion and density gradient centrifugation. The rat HSC strain IG12 was established by limiting dilution. In vitro changes of rALR, oxymatrine and salvia miltiorrhiza on IG12 acid phosphatase activity and extracellular matrix synthesis were investigated. Results Both rALR, matrine and salvia miltiorrhiza could significantly inhibit the proliferation of IG12 and selectively inhibit the synthesis of extracellular matrix. Conclusion Both rALR, oxymatrine and salvia miltiorrhiza have the anti-hepatic fibrosis effect; IG12 is of great value in the screening of anti-hepatic fibrosis drugs.