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实验取健康人O型血,加入抗人CD3单抗辅以少量的rIL-2诱导而形成CD3单克隆抗体激活的杀伤细胞(CD3AK),在培养的第4天至16天,每隔3天分别用MTT法测定杀伤活性一次,观察其体外抗肿瘤活性的动态变化,并与LAk、NK细胞比较,结果表明:(1)培养到第4天的CD3AK细胞已有明显的杀伤活性,杀伤率为39.15%,第10天达到高峰,杀伤率为81.54%,随后开始下降,第16天已下降为57.71%,(2)在同等条件下培养到第10天的CD3AK
The healthy human type O blood was taken and anti-CD3 monoclonal antibody was added to supplement with a small amount of rIL-2 to induce the formation of CD3 monoclonal antibody-activated killer cells (CD3AK) on the 4th to 16th days of culture, every 3 days. The activity of anti-tumor activity in vitro was measured by MTT method once, and compared with LAk and NK cells. The results showed that: (1) CD3AK cells cultured on the 4th day had obvious killing activity, killing rate It was 39.15%, peaked on the 10th day, the kill rate was 81.54%, then it began to decline, and it had dropped to 57.71% on the 16th day. (2) The CD3AK was cultivated on the 10th day under the same conditions.