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目的了解淋病奈瑟菌毒力岛(PAIs)在质粒及染色体上的分布情况。方法采用碱裂解法提取63株淋病奈瑟菌的质粒及染色体DNA,PCR扩增各PAIs基因(atlA,traG,traH)。结果共检出4.2kb,7.4kb,39.5kb,42.5kb四种质粒,总检出率为88.89%(56/63);以63株淋病奈瑟菌的染色体DNA为模板进行PCR扩增,atlA,traG和traH的阳性检出率分别为74.60%(47/63),34.92%(22/63)和68.25%(43/63);以56株淋病奈瑟菌的质粒DNA为模板进行PCR扩增,atlA,traG和traH的阳性检出率分别为75.00%(42/56),62.50%(35/56)和64.29%(36/56)。方法淋病奈瑟菌的毒力岛基因不仅存在于染色体上,而且存在于质粒上,且携带率高,分布广泛。
Objective To understand the distribution of plasmids and chromosomes on Neisseria gonorrhoeae virulence islands (PAIs). Methods Plasmids and chromosomal DNA of 63 strains of Neisseria gonorrhoeae were extracted by alkaline lysis, and the PAIs genes (atlA, traG, traH) were amplified by PCR. Results Four plasmids of 4.2kb, 7.4kb, 39.5kb and 42.5kb were detected with a total detection rate of 88.89% (56/63). 63 chromosomal DNA of Neisseria gonorrhoeae was used as a template for PCR amplification and atlA , and the positive rates of traG and traH were 74.60% (47/63), 34.92% (22/63) and 68.25% (43/63), respectively. The PCR products of 56 strains of Neisseria gonorrhoeae The positive detection rates of atlA, traG and traH were 75.00% (42/56), 62.50% (35/56) and 64.29% (36/56), respectively. Methods The virulence island genes of Neisseria gonorrhoeae not only exist on the chromosome, but also exist on the plasmids, with high carrying rate and wide distribution.