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目的 :研究脂蛋白酯酶、肝酸酶在兔胆囊结石中对血脂代谢的作用。方法 :采用日本杂交大耳兔 5 0只 ,随机分为对照组 ,1、 2、 3、 4周组为实验组 ,实验组饲以 1 2 %高胆固醇膳食诱发胆囊结石 ,测定各组脂蛋白酯酶、肝酯酶、血清脂质的动态变化 ,脂蛋白酯酶和肝酯酶采用比色法测定 ,血清脂质采用酶法测定。结果 :随着高胆固醇膳食进食时间延长 ,2周组、 3周组和 4周组分别有 4/10 ,6 /10和 7/10只动物出现胆囊结石 ;肝素化血清中脂蛋白酯酶和肝酯酶活性增加 ,以 3周组和 4周组升高明显 (与对照组比较 ,P <0 0 5 ) ;血清总胆固醇 ,甘油三酯 ,磷脂 ,低密度脂蛋白胆固醇和极低密度脂蛋白胆固醇逐渐明显升高 (与在对照组比较P <0 0 5 ) ,高密度脂蛋白胆固醇及其亚组份有降低趋势 ,但与对照组比较差异无显著性 (P <0 0 5 )。结论 :高胆固醇膳食后 ,脂蛋白酯酶和肝酯酶活性增强 ,致血脂代谢异常变化 ,促进了胆囊结石形成
Objective: To study the effect of lipoprotein lipase and hepatic enzyme on blood lipid metabolism in rabbit gallbladder stones. Methods: Fifty Japanese hybrid rabbits were randomly divided into control group, 1, 2, 3, 4 weeks group as experimental group, experimental group fed with 12% cholesterol diet induced gallstone, the determination of lipoprotein Esterase, hepatic esterase, serum lipid dynamic changes, lipoprotein lipase and hepatic esterase using colorimetric assay, serum lipid enzymatic assay. Results: With hypercholesterolemia, gallstone was found in 4/10, 6/10, and 7/10 animals in the 2nd week, 3rd week, and 4th week groups respectively. The levels of lipoprotein lipase and Liver esterase activity increased significantly in 3-week and 4-week groups (P <0.05 compared with the control group); serum total cholesterol, triglycerides, phospholipids, low density lipoprotein cholesterol, and very low density lipoprotein cholesterol (P <0.05), while the levels of HDL cholesterol and its subcomponents decreased. However, there was no significant difference between the control group and the control group (P <0.05). Conclusion: After high cholesterol diet, lipoprotein esterase and liver esterase activity increased, resulting in abnormal changes in blood lipid metabolism, and promote the formation of gallstones