自然界中依赖烟酰胺类辅酶(NAD+或NADP+)的脱氢酶是氧化还原酶中最重要的一类,基于此类酶的生物传感器应用前景广阔,近年来发展迅速。构建这类传感器需要两项关键技术,即氧化型辅酶在电极表面的再生和辅酶固定化。介绍了辅酶电化学再生的主要方法、辅酶固定化的常见手段,以及相关的研究进展。 In nature, the dehydrogenase that depends on nicotinamide coenzyme (NAD + or NADP +) is the most important type of redox enzyme. The biosensor based on this kind of enzyme has a bright future and has been rapidly developed in recent years. Two key technologies are needed to construct this type of sensor: the regeneration of oxidized coenzyme on the electrode surface and the coenzyme immobilization. Introduced the main method of coenzyme electrochemical regeneration, coenzyme immobilization common means, and the related research progress.