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目的观察灵芝多糖(GLP)对阿尔茨海默病(AD)大鼠海马组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量以及空间学习记忆能力等的影响。方法双侧海马内一次性注射β-淀粉样多肽25~35片段(Aβ25~35)制作大鼠AD模型,腹腔注射灵芝多糖水溶液,1周后进行Morris水迷宫检测大鼠空间学习记忆能力变化,采用分光光度法测定大鼠海马组织SOD活性和MDA含量,采用透射电镜观察海马神经元的超微结构变化,以及灵芝多糖对上述各指标的影响。结果灵芝多糖能明显改善AD模型大鼠低下的空间学习记忆能力,显著提高模型大鼠海马组织SOD活性及降低MDA含量,而且灵芝多糖能明显改善模型大鼠脑组织海马CAl区神经元的退行性变化。结论灵芝多糖能提高海马内抗氧化酶SOD活性,降低丙二醛MDA含量,改善海马CA1区神经元的退行性变化,对老年性痴呆大鼠学习记忆能力可能有增强和提高作用。
Objective To observe the effects of Ganoderma lucidum polysaccharides (GLP) on SOD activity, MDA content and spatial learning and memory ability of hippocampus in Alzheimer’s disease (AD) rats. METHODS: A rat model of AD was produced by intraperitoneal injection of β-amyloid peptide 25-35 fragment (Aβ25-35) into the bilateral hippocampus. The rats were intraperitoneally injected with Ganoderma lucidum polysaccharide solution. One week later, the Morris water maze was used to detect spatial learning and memory changes in rats. Spectrophotometry was used to determine the activity of SOD and MDA in rat hippocampus. Transmission electron microscopy was used to observe the ultrastructural changes of hippocampal neurons and the effects of ganoderma lucidum polysaccharide on the above indexes. RESULTS Ganoderma lucidum polysaccharide could significantly improve the spatial learning and memory ability of AD rats, significantly increase the activity of SOD and decrease the content of MDA in hippocampus of model rats. Moreover, Ganoderma lucidum polysaccharide can obviously improve the neuronal degeneration of rat hippocampal CA1 region. Variety. Conclusion Ganoderma lucidum polysaccharides can increase SOD activity in hippocampus, decrease malondialdehyde (MDA) content, and improve degenerative changes of neurons in hippocampal CA1 region. It may enhance and improve learning and memory ability of Alzheimer’s disease in rats.