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目的观察多潘立酮对增强消化间期移行性复合运动(MMC)及促进胃窦十二指肠协调运动的有效性。方法在体实验:将30只Wister大鼠均分为对照(生理盐水)组、多潘立酮组和莫沙必利组。在各组大鼠胃窦、幽门和十二指肠埋植应力传感器,记录MMC及测定胃窦十二指肠协调运动。离体实验:应用血管灌流(Krebs-Ringer液、多潘立酮、阿托品+多潘立酮、河豚毒素+多潘立酮或莫沙必利)大鼠离体胃十二指肠制备。在胃窦和十二指肠缝一应力传感器,记录胃和十二指肠运动及测定胃窦十二指肠协调性。结果在体实验:①清醒大鼠消化间期出现典型MMCⅠ、Ⅱ、Ⅲ、Ⅳ相。②多潘立酮可明显增强MMC的收缩运动,使胃窦和十二指肠平均振幅分别比对照组增加85.1%±11.4%和83.0%±6.3%。③多潘立酮可明显增加MMC胃窦十二指肠协调运动,由胃窦引发的十二指肠收缩波比对照组增加91.2%±9.4%。离体实验:①血管灌流多潘立酮可显著刺激离体胃十二指肠运动和胃窦十二指肠协调运动,分别较Krebs-Ringer液组增加66.2%±12.1%和76.5%±5.8%。②阿托品与河豚毒素可阻断多潘立酮兴奋胃十二指肠运动及胃窦十二指肠协调性的作用。结论多潘立酮可明显增加胃窦十二指肠动力及协调运动。多潘立酮促进胃肠动力和协调运动的作用机制,除已知阻断外周多巴胺受体外,还可能通过肠神经系统内胆碱能神经介导。
Objective To observe the efficacy of domperidone in enhancing duodenal metaplasia (MMC) and promoting duodenal uncoordinated movement in the antrum. Methods In vivo experiments: 30 Wister rats were equally divided into control (saline) group, domperidone group and mosapride group. Stress sensors were placed in the gastric antrum, pylorus and duodenum of rats in each group, MMC was recorded and the duodenum of gastric antrum was measured for coordinated movement. In vitro experiments: Gastrodotomy (Krebs-Ringer’s solution, domperidone, atropine + domperidone, tetrodotoxin + domperidone or mosapride) rats were prepared in vitro gastroduodenal. Suture a stress sensor in the antrum and duodenum, record gastric and duodenal motility and determine gastric duodenal duodenal coordination. Results In vivo experiment: ① The typical MMC Ⅰ, Ⅱ, Ⅲ and Ⅳ phases appeared in the digestive tract of conscious rats. ② Domperidone can significantly enhance the contractile activity of MMC, so that the average amplitude of gastric antrum and duodenum were increased by 85.1% ± 11.4% and 83.0% ± 6.3% respectively compared with the control group. Domperidone can significantly increase MMC duodenum anastomosis gastric antrum induced duodenal contraction wave than the control group increased 91.2% ± 9.4%. Ex vivo experiments: (1) Perfial in vascular perfusion significantly stimulated the gastroduodenal movement in vitro and the duodenal anastomosis in gastric antrum, which increased by 66.2% ± 12.1% and 76.5% ± 5.8% respectively compared with Krebs-Ringer liquid group. ② atropine and tetrodotoxin can block the domperidone excited gastroduodenal movement and gastric duodenal coordination role. Conclusion Domperidone can significantly increase the duodenal motility and coordination of gastric antrum. Domperidone can promote gastrointestinal motility and coordinate the mechanism of action, in addition to blocking the peripheral dopamine receptors, but also may be mediated through the cholinergic nerve in the enteric nervous system.