目的　通过牛磺酸对新生大鼠心肌成纤维细胞增殖和胶原合成及对Ⅰ /Ⅲ型胶原比值的影响 ,从细胞水平探讨其在体外抑制心肌重塑的作用。方法　在培养的心肌成纤维细胞中加入不同浓度的牛磺酸 ,用羟脯氨酸法测定胶原量 ,MTT比色法检测细胞增殖情况 ,SDS PAGE电泳测胶原Ⅰ /Ⅲ型比值。结果　 10 0mmol/L、2 0 0mmol/L牛磺酸用药 2 4h和 4 8h及 5 0mmol/L用药 72h后能明显抑制心肌成纤维细胞的增殖及胶原的合成 ,Ⅰ /Ⅲ型胶原比值明显下降。结论　牛磺酸能通过抑制心肌成纤维细胞增殖和胶原合成 ,降低Ⅰ /Ⅲ型胶原比值起到抗心肌重塑的作用。 OBJECTIVE: To investigate the effect of taurine on the proliferation and collagen synthesis of neonatal rat cardiac fibroblasts and the ratio of type Ⅰ / Ⅲ collagen, and to explore its role in inhibiting myocardial remodeling in vitro. Methods Different concentrations of taurine were added into the cultured cardiac fibroblasts. The hydroxyproline method was used to determine the amount of collagen. MTT assay was used to detect the cell proliferation. SDS - PAGE was used to determine the collagen type Ⅰ / Ⅲ ratio. Results The proliferation and collagen synthesis of cardiac fibroblasts were significantly inhibited by 10 mmol / L, 200 mmol / L taurine for 24 h, 48 h and 50 mmol / L, and the ratio of type Ⅰ / Ⅲ collagen was significantly decreased . Conclusion Taurine can play a role in anti-myocardial remodeling by inhibiting the proliferation and collagen synthesis of cardiac fibroblasts and decreasing the ratio of type Ⅰ / Ⅲ collagen.