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本文比较了不同酶液、渗透稳定剂、酶液pH、酶解温度、菌丝生长培养基成份及菌龄等因素对原生质体形成的作用及影响。用1.5％粗纤维素酶或1％脱壁酶、0.7MNaCl为渗透稳定剂,温度32℃、pH6、菌丝生长培养基为Sabouraud琼脂培养基,菌龄16小时,可以从所试的6株菌株的菌丝体得到大量的原生质体（4.0×10~6个/ml）。0.5MNaCl为稳定液,黄豆粉培养基上原生质体再生率可达11.4％。对原生质体释放和再生的形态学过程进行了描述和显微摄影。
In this paper, the effects of different enzyme solutions, osmotic stabilizers, enzyme pH, enzymolysis temperature, mycelial growth medium composition and bacterial age on protoplast formation were compared. With 1.5% crude cellulase or 1% wall-removing enzyme, 0.7M NaCl as osmotic stabilizer, temperature 32 ℃, pH6, mycelium growth medium Sabouraud agar medium, bacterial age 16 hours, from the six strains tested The mycelium of the strain obtained a large amount of protoplasts (4.0 × 10 -6 / ml). 0.5MNaCl as a stable solution, soybean powder medium protoplast regeneration rate of up to 11.4%. Morphological processes of protoplast release and regeneration were described and microscopically photographed.