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抗生素对人体免疫的影响已成为许多学者的研究课题。目前已经研究的抗菌剂有:利福平、四环素、羧苄青霉素、青霉素、呋喃呾啶、几种头孢菌素和一些抗霉菌剂。喹诺酮类在治疗多种感染性疾病中,显示为一类很有希望的抗菌药物,但这类化合物对细胞免疫反应的影响尚有争论。本研究采用Ficoll-hypaque沉淀法,从健康青年志愿者的肝素化全血中取得单核细胞,配制成5×10~5单核白细胞/毫升浓度,进行细胞培养。对照组仅有组织培养液,而实验组含有:Amifloxacin、丙氟哌酸、氟哌酸或罗索沙新(Rosoxacin),药物浓度范围为0。1～10mg/L。在细胞培养的第一天加入
The impact of antibiotics on human immunity has become the subject of many scholars. Antibacterial agents that have been studied so far include: rifampin, tetracycline, carbenicillin, penicillin, furanidinium, several cephalosporins, and some antimycotic agents. Quinolones appear to be a promising class of antimicrobials in the treatment of a variety of infectious diseases, but the impact of these compounds on cellular immune responses remains controversial. In this study, Ficoll-hypaque precipitation method was used to obtain mononuclear cells from heparinized whole blood of healthy young volunteers, and the cells were cultured in the concentration of 5 × 10 -5 mononuclear leukocytes / ml. The control group consisted of only tissue culture fluid, whereas the experimental group contained Amifloxacin, Ciprofloxacin, Norfloxacin or Rosoxacin with drug concentrations ranging from 0.1 to 10 mg / L. Add on the first day of cell culture