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以贵州喀斯特地区10个古茶树扦插苗为材料,采用盆栽控水法研究其对干旱胁迫的生理响应,并筛选出抗旱的种质资源。试验结果表明,随着干旱胁迫程度的加重,10个古茶树扦插苗的相对含水量、Fv/Fm、气孔开放率和气孔大小均呈下降趋势,而相对电导率、丙二醛含量、H2O2含量与产生速率以及气孔密度呈上升趋势;从10个古茶树扦插苗中选出1个强抗旱材料,5个较强抗旱材料。田间持续干旱试验验证了基于生理指标的隶属函数分析对于古茶树扦插苗筛选的准确性及可靠性。
Ten ancient tea tree cuttings in karst area of Guizhou Province were used as materials to study the physiological response to drought stress by potted water control method and to screen drought-resistant germplasm resources. The results showed that with the aggravation of drought stress, the relative water content, Fv / Fm, stomatal opening and stomatal size of the cuttings of 10 ancient tea plants all showed a decreasing trend, while the relative conductivity, MDA content, H2O2 content With the production rate and stomatal density increased, one strong drought-resistant material and five strong drought-resistant materials were selected from 10 ancient tea tree cutting seedlings. Field continuous drought test validated the accuracy and reliability of the screening based on physiological index membership function analysis.