目的 :建立贯叶连翘中金丝桃素的测定方法。方法 :采用紫外可见光谱测试 ,在 589nm处有特征吸收。结果 :样品的平均加样回收率为 99.2 7% ,精密度及稳定性的相对标准偏差分别为 0 .1 9%及 0 .55%。结论 :本法可以作为企业生产贯叶连翘提取物的质量控制测试方法。 Objective : To establish a method for the determination of hypericin in Hypericum perforatum. Method: UV-Vis spectroscopy was used to detect the characteristic absorption at 589nm. Results: The average sample recovery was 99.27%, and the relative standard deviations of precision and stability were 0.19% and 0.55%, respectively. Conclusion: This method can be used as a quality control test method for the production of St. John’s wort extracts.