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用2个抗性基因同源序列(RGA)引物分析了菲律宾国家水稻所水稻种质资源库中265个抗或感水稻东格鲁病(RTV)的种质资源的遗传差异。引物CLRR的分辨能力极高,共扩增出了48条多态带,平均每个品种28.6条。每个品种平均扩增出46.9条多态带。DNA指纹图谱分析结果表明,通过变性聚丙烯酰胺凝胶电泳,RGA引物能有效地分析群体材料在分子遗传上的差异,这说明RGA标记是水稻种质资源DNA指纹快速识别的理想选择。通过聚类分析还发现本研究的水稻品种间存在很大的遗传差异。
The genetic diversity of 265 resistant or susceptible rice tungross disease (RTV) germplasms in the rice germplasm resources database of the National Rice Institute of Philippines was analyzed using two resistance gene homologous sequences (RGA) primers. Primer CLRR resolution is very high, a total of 48 polymorphic bands were amplified, an average of 28.6 varieties per species. An average of 46.9 polymorphic bands were amplified for each variety. The results of DNA fingerprinting indicated that RGA primers could effectively analyze the molecular genetic differences among population materials by denaturing polyacrylamide gel electrophoresis, which indicated that RGA markers were the ideal choice for DNA fingerprinting in rice germplasm resources. Cluster analysis also showed that there was a large genetic difference between the rice varieties in this study.