论文部分内容阅读
目的 :探讨鼻咽癌组织中 p5 3、bcl- 2、EBV表达情况及与主要临床指标间关系。方法 :免疫组化检测p5 3、bcl- 2蛋白 ,多聚酶链反应 (PCR)检测 EBV。结果 :慢性炎性组织中 p5 3、bcl- 2及 EBV阳性率分别为 7.7%、2 3.1%和 7.7% ,明显低于鼻咽癌组织中的阳性率 (分别为 75 .9%、88.9%和 87.0 % ) (P<0 .0 1)。三者在鼻咽癌组织中共同阳性率为 72 .2 % ,p5 3、bcl- 2明显正相关 (r =0 .78,P <0 .0 1)。鼻咽癌伴颈淋巴结转移组 p5 3阳性率(90 .0 % )明显高于无颈淋巴结转移组 (5 8.3% ) (P <0 .0 1) ,临床 、 和 期 p5 3阳性率 (分别为 92 .9%、78.9%和 10 0 % )均明显高于临床 期 (38.5 % ) (P <0 .0 1) ,且表达强度有增加的趋势。结论 :p5 3、bcl- 2及 EBV可作为鼻咽癌早期诊断的辅助指标之一 ,p5 3阳性的鼻咽癌患者较易发生颈淋巴结转移 ,随着鼻咽癌病程的发展 ,p5 3表达率及表达强度均增加 ,三者在鼻咽癌发生发展中起着重要作用。
Objective: To investigate the expression of p5 3, bcl-2 and EBV in nasopharyngeal carcinoma (NPC) and its relationship with the main clinical parameters. Methods: The expressions of p53 and bcl-2 were detected by immunohistochemistry and EBV by polymerase chain reaction (PCR). Results: The positive rates of p53, bcl-2 and EBV in chronic inflammatory tissues were 7.7%, 21.1% and 7.7%, respectively, which were significantly lower than those in nasopharyngeal carcinoma (75.9%, 88.9% And 87.0%) (P <0.01). The positive rate of the three in nasopharyngeal carcinoma was 72.2%, p5 3 and bcl-2 were positively correlated (r = 0.78, P <0.01). The positive rate of p5 3 in nasopharyngeal carcinoma with cervical lymph node metastasis (90.0%) was significantly higher than that in no cervical lymph node metastasis (53.3%) (P <0.01), and the positive rate of p5 3 (92.9%, 78.9% and 100%) were significantly higher than the clinical stage (38.5%) (P <0.01), and the expression intensity had an increasing tendency. Conclusions: p5 3, bcl-2 and EBV may serve as one of the auxiliary indicators for early diagnosis of nasopharyngeal carcinoma. Patients with p5 3 -positive nasopharyngeal carcinoma are more likely to have cervical lymph node metastasis. With the progression of nasopharyngeal carcinoma, the expression of p5 3 Rate and expression intensity are increased, the three play an important role in the occurrence and development of nasopharyngeal carcinoma.