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目的探讨S100钙结合蛋白A12(S100A12)在早产孕妇外周血、脐血及胎膜组织中的表达及其与亚临床绒毛膜羊膜炎(subclinical chorioamnionitis,HCA)的关系。方法选择早产临产孕妇46例,根据产后胎膜病理结果是否合并亚临床绒毛膜羊膜炎,分为感染组(n=21)与未感染组(n=25)。采用ELISA法检测两组孕妇外周血、脐血中S100A12水平;逆转录RT-PCR法检测胎膜组织中S100A12 m RNA表达水平。结果感染组孕妇外周血、脐血、胎膜组织中S100A12水平[(173.30±18.92)、(105.71±8.72)、(1.38±0.37)]与未感染组[(138.26±11.02)、(88.26±7.00)、(1.07±0.15)]比较,差异有统计学意义(P<0.05);感染组孕妇外周血中S100A12水平与脐血及胎膜组织中S100A12水平均呈正相关(r=0.861,0.883;P<0.05);感染组新生儿感染发病率(86%,18/21)高于未感染组(56%,14/25)(P<0.05)。结论孕妇外周血、脐血、胎膜组织中S100A12表达水平升高,可能与合并亚临床绒毛膜羊膜炎及新生儿感染有关。
Objective To investigate the expression of S100 calcium binding protein A12 (S100A12) in peripheral blood, cord blood and fetal membranes in pregnant women with premature labor and its relationship with subclinical chorioamnionitis (HCA). Methods Forty-six pregnant women undergoing preterm delivery were divided into infection group (n = 21) and uninfected group (n = 25) according to whether there was any subclinical chorioamnionitis in the prenatal and fetal membranes. The levels of S100A12 in peripheral blood and umbilical cord blood were detected by ELISA. The expression of S100A12 mRNA in fetal membranes was detected by RT-PCR. Results The levels of S100A12 in the peripheral blood, umbilical cord blood and fetal membranes in the infected group were significantly higher than those in the uninfected group ([138.36 ± 11.02], (88.26 ± 7.00), [(173.30 ± 18.92) ), (1.07 ± 0.15)], the difference was statistically significant (P <0.05). The level of S100A12 in peripheral blood of pregnant women was positively correlated with the level of S100A12 in cord blood and fetal membranes (r = 0.861,0.883; P <0.05). The incidence of neonatal infection in the infected group (86%, 18/21) was higher than that in the non-infected group (56%, 14/25) (P <0.05). Conclusion The expression of S100A12 in peripheral blood, umbilical cord blood and fetal membranes of pregnant women may be related to subclinical chorioamnionitis and neonatal infection.