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交感神经节中有脑啡肽神经末梢,可能以突触前抑制的方式抑制胆硷能神经的传递。Konishi等在成年豚鼠游离的交感肠系膜下神经节中进行了实验性研究。用细胞内记录法记录神经节细胞的EPSP_S。通过一对电极给一小束肠系膜下神经(节前纤维)试验刺激,以诱发快的EPSP_S,而对其余的节前纤维,通过另外的电极给予条件刺激。肠系膜下神经节中的脑啡肽神经末梢是节前纤维,给条件刺激(50Hz,8秒)以后,试验刺激引起的EPSP的平均值减小,约为条件刺激前试验刺激引起的EPSP的50%,抑制持续2～4分钟。纳络酮(1～3μM)可以清除条件性节前纤维刺激对胆硷能EPSP的抑制效应,相同浓度的纳络酮也几乎可完全阻断作用很强的脑啡肽拟似物[D-Al_α~2]-甲-脑啡肽(0.3～2μM)对胆硷能EPSP_S的抑制作用。纳
There are enkephalin nerve terminals in the sympathetic ganglia that may inhibit the transmission of cholinergic nerves in a presynaptic manner. Konishi et al. Conducted experimental studies in the sympathetic inferior mesenteric ganglion of adult guinea pigs. Intracellular recording method of recording ganglion cell EPSP_S. A small bundle of inferior mesenteric nerve (preganglionic) experiments were stimulated with a pair of electrodes to induce fast EPSP_S, while the remaining preganglionic fibers were conditionally stimulated with additional electrodes. The enkephalin nerve endings in the inferior mesenteric ganglion are preganglionic fibers. After the conditioned stimulus (50 Hz, 8 seconds), the mean value of the EPSP induced by the test stimulus is reduced to about 50% of the EPSP induced by the pre-stimulus test stimulus %, Inhibition lasting 2 to 4 minutes. Naloxone (1 ~ 3μM) can clear the inhibitory effect of conditional prenatal fiber stimulation on cholinergic EPSP, and naloxone with the same concentration can almost completely block the enkephalin analogue [D- Al_α ~ 2] - A - enkephalin (0.3 ~ 2μM) on cholinergic EPSP_S inhibition. Satisfied