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分别在水溶液中和 Ｗ ／Ｏ型 ＣＴＡＢ／正戊醇／异辛烷 ／水微乳液体系中研究了测定ＳＯＤ酶活性的邻苯三酚法，发现在反向微乳液中，“小水池”的低介电常数和ｐＨ抑制了底物超氧化物阴离子Ｏ２－·的产生．只有提高外加缓冲液的ｐＨ或“小水池”的水化度，才能得到所需浓度的Ｏ２－·，从而初步解决了在微乳液中ＳＯＤ作用废物的产生问题，为在微乳液中准确测定ＳＯＤ酶活性创造了条件．
Pyrogallol assay was used to determine the activity of SOD in aqueous solution and W / O CTAB / n-pentanol / isooctane / water microemulsion respectively. It was found that in the reverse microemulsion, “small pool” Low dielectric constant and pH inhibit the production of the substrate superoxide anion O2-. Only by raising the pH of the external buffer or the degree of hydration of the “small pool”, the desired concentration of O2- · can be obtained, so as to initially solve the problem of the generation of the SOD waste in the microemulsion. In order to accurately measure the SOD Enzyme activity created the conditions.