分离出的3株疟原虫是:西非1株、利比里亚1株和海地1株。均用肝素抗凝取病人血,放在湿冰中,然后携回实验室,按Trager-Jensen的体外烛缸培养法对这3株疟原虫进行体外连续培养。培养的结果,西非1株明显不同于利比里亚1株和海地1株,西非1株进行体外培养时疟原虫不需要一个适应期,从培养的第1天起,疟原虫数量 Three isolates of Plasmodium were: 1 in West Africa, 1 in Liberia and 1 in Haiti. All patients were treated with anticoagulant heparin, placed in wet ice, and then brought back to the laboratory. The three plasmodium strains were continuously cultured in vitro according to Trager-Jensen’s in vitro candling culture. As a result, one strain in West Africa was significantly different from the one in Liberia and one in Haiti. Plasmodium did not need an adaptation period when in vitro culture of one strain in West Africa. From the first day of culture, the number of malaria parasites