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目的 :探讨 3p2 4染色体位点的杂和缺失和 11p15 .5位点的点突变同食管癌之间的相关性 ,寻找这两个位点中潜在的抑癌基因 ,并希望通过此研究能为最后确定该基因的功能奠定基础。 方法 :用 PCR- RFL P法检测3p2 4染色体的三个位点 (EAβ MD、EAβ H、EAβ R)上等位基因的杂合缺失情况 ,PCR- SSCP法检测 11p15 .5位点的点突变。 结果 :经 RFL P法显示 :在 3p2 4的 EAβ MD位点 ,杂合缺失率为 75 % ,EAβ H位点的杂合缺失率为5 0 % ,EAβ R位点的杂合缺失率为 6 .2 5 % ;SSCP法未发现 11p15 .5位点存在点突变。 结论 :EAβ MD和 EAβ H位点的杂合缺失率较高 ,此两位点可能存在抑癌基因 ,其突变可能与食管癌的发生有关
OBJECTIVE: To investigate the association between the missense deletion of 3p2 4 locus and the point mutation of 11p15.5 locus in esophageal cancer, and to search for potential tumor suppressor genes in these two loci. Finally determine the function of this gene laid the foundation. Methods: The heterozygous deletions of alleles at 3p2 4 sites (EAβ MD, EAβ H, EAβ R) were detected by PCR-RFLp. The point mutation at 11p15.5 was detected by PCR-SSCP . Results: The frequency of heterozygosity deletion was 75% in the EAβ MD site of 3p2 4, 50% in the heterozygous EAβ H site, and 6 in the heterozygous EAβ R site .2 5%; SSCP method did not find point 11p15. 5 point mutation. Conclusion: The heterozygous deletion rate of EAβ MD and EAβ H loci is high, and there may exist tumor suppressor genes in these two loci, which may be related to the occurrence of esophageal cancer