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目的:研究赤芍药材经钴-60射线辐照前后有效成分的含量变化情况。方法:采用HPLC方法,色谱柱为瑞士SPHERI-GEL ODS C18(150mm×4.6mm,5μm)柱,流动相为甲醇-水,梯度洗脱(0～10min,15%→20%甲醇;10～30min,20%→30%甲醇),流速1.0mL·min-1,检测波长为260.5nm,柱温25℃,分析时间为30min。结果:赤芍药材经钴-60射线辐照后主要有效成分降低近19%。结论:经过系统的方法学考察,该方法具有简便、稳定、可重复性强等特点。可作为赤芍药材辐照前后芍药苷含量变化的评价依据,同时在处理含赤芍药材的中药制剂时应考虑到辐照对主成分的影响。
OBJECTIVE: To study the changes of active ingredients in Radix Paeoniae Rubra before and after cobalt-60 radiation. Methods: The HPLC method was used on a SPHERI-GEL ODS C18 column (150 mm × 4.6 mm, 5 μm) with a mobile phase of methanol-water and gradient elution (0-10 min, 15% → 20% methanol; , 20% → 30% methanol), the flow rate was 1.0mL · min-1, the detection wavelength was 260.5nm, the column temperature was 25 ℃ and the analysis time was 30min. Results: The main active ingredients of Radix Paeoniae Rubra were reduced by nearly 19% after cobalt-60 radiation. Conclusion: After systematic investigation, this method is simple, stable and reproducible. It can be used as the basis for the evaluation of the change of paeoniflorin before and after Radix Paeoniae Rubra Radiat. At the same time, the effects of irradiation on the main components should be considered when dealing with the traditional Chinese medicine preparations containing Radix Paeoniae Rubra.