论文部分内容阅读
目的 建立正常人垂体组织基因表达谱。方法 垂体cDNA文库构建、大规模表达序列标记 (EST)测序及生物信息学处理。结果 在所测的 72 2 2条可分析的EST中 ,2 949条 (4 1% )为已知基因 ,其中参与基因 /蛋白表达者最高 (2 3.2 % ) ,13 .7%的基因与内分泌相关 ,18.1%的基因参与信号转导。前 2位高表达的基因是促生长素和催乳素。垂体表达甘丙肽、可卡因和安非他明调节的转录肽 (CART)及黑色素浓集激素 (MCH)等与食欲调节有关的激素。还表达与免疫有关的细胞因子和受体。Pit 1、锌指蛋白 (ZNF) 74和PLAG1在垂体中是相对特异表达的。结论 正常垂体组织中参与信号转导的基因明显高于心脏和CD34 +造血前体细胞 ,而与脑、下丘脑、肾上腺组织相近。甘丙肽和CART可能是参与食欲调节的垂体激素。垂体和免疫系统之间存在广泛的相互作用。Pit 1、ZNF74和PLAG1可能是垂体特异表达的转录因子。
Objective To establish a normal human pituitary tissue gene expression profile. Methods Pituitary cDNA library construction, large-scale expression sequence tagging (EST) sequencing and bioinformatics processing. Results Among the 7222 ESTs that could be analyzed, 2 949 (41%) were known genes, of which the highest was involved in gene / protein expression (22.2%), 13.7% was associated with endocrine Related, 18.1% of the genes involved in signal transduction. The first two highly expressed genes are somatotropin and prolactin. Pituitary gonadotropin, cocaine and amphetamine-regulated transcriptional (CART) and melanin-concentrating hormone (MCH) and other appetite-regulated hormones. Also expressed immune-related cytokines and receptors. Pit 1, zinc finger protein (ZNF) 74 and PLAG1 are relatively specific in the pituitary gland. Conclusion The genes involved in signal transduction in normal pituitary tissue were significantly higher than those in cardiac and CD34 + hematopoietic precursor cells, but similar to those in brain, hypothalamus and adrenal gland. Galanin and CART may be involved in the regulation of appetite pituitary hormone. There is a wide range of interactions between the pituitary gland and the immune system. Pit 1, ZNF74 and PLAG1 may be pituitary-specific transcription factors.