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目的:研究气相色谱法测定肉苁蓉多糖中单糖组分的方法。方法:采用单因素实验,考察各因素对肉苁蓉多糖水解的影响;采用气相色谱法测定肉苁蓉多糖中的单糖组成。结果:肉苁蓉多糖水解的最佳条件为硫酸浓度3.0 mol/L,水解时间8 h和水解温度110℃;各单糖精密度RSD为0.92%~4.68%,重复性RSD为0.92%~1.77%,稳定性RSD为1.61%~3.61%,加样回收率为99.3%~100.3%,精密度、重复性、稳定性和回收率良好;肉苁蓉多糖主要由甘露糖、葡萄糖和半乳糖组成,摩尔比为2.01∶5.72∶2。结论:此法适用于肉苁蓉多糖中单糖组成分析。
Objective: To study the method for the determination of monosaccharide composition in Cistanche desertica by gas chromatography. Methods: Single factor experiments were conducted to investigate the effect of various factors on the polysaccharide hydrolysis of Cistanche desertica. The monosaccharide composition of Cistanche desertica polysaccharides was determined by gas chromatography. Results: The optimum conditions for the hydrolysis of Cistanche desertica were: sulfuric acid concentration 3.0 mol / L, hydrolysis time 8 h and hydrolysis temperature 110 ℃. The RSDs of all the polysaccharides were 0.92% -4.68%, and the repeatability RSD was 0.92% -1.77% The RSD was 1.61% -3.61%, the recoveries were 99.3% ~ 100.3%, the precision, repeatability, stability and recovery were good. Cistanche desertica polysaccharides mainly consisted of mannose, glucose and galactose with the molar ratio of 2.01 : 5.72: 2. Conclusion: This method is suitable for the analysis of monosaccharide in Cistanche desertica polysaccharides.