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目的报告一起由霍乱弧菌引起的食源性暴发疫情的病原学特征。方法在流行病学调查、临床表现基础上开展了实验室细菌分离培养和病原学鉴定。结果采集聚餐者肛拭标本52份、外环境检索样本49份,其中10份肛拭标本和1份井水检出O1群霍乱弧菌小川型,噬菌体-生物分型为15 f,毒力基因ctx阳性。48份密切接触者标本中未检出霍乱弧菌。11株霍乱弧菌生化鉴定结果完全一致,氧化酶试验、鸟氨酸脱羧酶、赖氨酸脱羧酶、葡萄糖、蔗糖阳性,精氨酸双水解酶、阿拉伯糖阴性。药敏试验:对阿莫西林、利福平中度敏感,对诺氟沙星、先锋噻肟、复方新诺明、庆大霉素、萘啶酸敏感性较强。结论本次食源性霍乱暴发疫情的病原菌是O1群霍乱弧菌小川型15 f,诺氟沙星、先锋噻肟等可为抗菌治疗和预防性服药的首选药物。
Objective To report the etiological characteristics of a foodborne outbreak caused by V. cholerae. Methods Based on epidemiological investigation and clinical manifestations, laboratory bacterial isolation and culture and etiological identification were carried out. Results A total of 52 swab samples from excrement collectors and 49 samples from external environment were collected. Among them, 10 swab samples and 1 well water were used to detect O1 cholera toxin type Ogawa, phage-typed to 15 f, virulence genes ctx positive. Vibrio cholera was not detected in 48 samples of close contacts. The results of biochemical identification of 11 strains of V. cholerae were identical. The oxidase test, ornithine decarboxylase, lysine decarboxylase, glucose, sucrose positive, arginine hydrolase and arabinose were negative. Susceptibility test: amoxicillin, rifampin moderately sensitive to norfloxacin, vanguard phenothiazole, cotrimoxazole, gentamicin, nalidixic acid sensitivity. Conclusion The pathogen of the outbreak of food-borne cholera outbreak is Vibrio cholera O1 Ogawa type 15 f, norfloxacin, vanguard and other drugs for the prevention and treatment of antibacterial drug of choice.