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目的：确定肾综合征出血热（ＨＦＲＳ）病毒抗原在尸检肝组织肝内胆管上皮细胞的定位．方法：免疫组织化学多重ＰＡＰ法．结果：１８例尸检肝组织中，４例肝内胆管发现ＨＦＲＳ病毒抗原，主要分布于肝内大胆管，小叶间胆管和小胆管的上皮细胞中．抗原阳性物质呈细颗粒状，定位于胆管上皮细胞核上区的胞质中．结论：陈旧尸检肝组织肝内胆管上皮细胞胞浆中存在ＨＦＲＳ病毒抗原．可能随胆汁排出在粪便中成为污染来源．
OBJECTIVE: To determine the localization of hemorrhagic fever with renal syndrome (HFRS) antigen on the liver intrahepatic bile duct epithelial cells at autopsy. Methods: Immunohistochemical multiplex PAP method. Results: Of the 18 autopsy liver tissues, 4 cases of intrahepatic bile duct were found to have antigens of HFRS virus, mainly distributed in the epithelial cells of intrahepatic duct, interlobular duct and cholangiocarcinoma. Antigen positive material was fine granular, located in the bile duct epithelial cells in the cytoplasm of the area. Conclusion: There are HFRS virus antigens in the cytoplasm of intrahepatic bile duct epithelial cells in old autopsy liver. May be discharged with the bile in the faeces as a source of pollution.