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目的:探讨人参皂苷Rg1对抗1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的C57BL小鼠黑质神经元凋亡的可能机制.方法:MPTP(30 mg·kg~(-1)·d~(-1)×5 d)腹腔注射制备C57BL小鼠帕金森病模型,同时预防组分别以不同剂量人参皂苷Rg1(2.5、5.0、10.0 mg·kg~(-1)·d~(-1)×8 d)于MPTP注射前预先腹腔注射小鼠.用Nissl染色和TH组化染色观察黑质损害情况,TUNEL染色检测细胞凋亡,同时运用免疫组织化学方法检测caspase-3的活性片段以及Bcl-2、Bcl-xl、Bax、iNOS和 nNOS的表达情况.结果:人参皂苷Rg1(5.0和10.0 mg/kg)预处理能使黑质致密带Nissl阳性神经元和TH阳性神经元的脱失减少,同时降低了TUNEL阳性率,并伴有Bcl-2和Bcl-xl表达增加,Bax和iNOS表达减少以及抑制caspase-3的激活.结论:人参皂苷Rg1预处理对MPTP诱导的小鼠黑质神经元凋亡有明显的保护作用,其作用可能是通过降低iNOS和Bax蛋白表达,增加Bcl-2和Bcl-xl蛋白表达以及抑制caspase-3的激活来实现的.
Objective: To investigate the possible mechanism of ginsenoside Rg1 against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced apoptosis of substantia nigra neurons in C57BL mice. Methods: MPTP (MPTP) 30 mg·kg -1 ·d -1 ×5 d were intraperitoneally injected into the Parkinson’s disease model of C57BL mice, and the preventive groups were given different doses of ginsenoside Rg1 (2.5, 5.0, 10.0 mg·kg, respectively). ~(-1)·d~(-1)×8 d) The mice were intraperitoneally injected before MPTP injection. Nissl staining and TH histochemistry staining were used to observe the damage of the substantia nigra. TUNEL staining was used to detect the apoptosis. Histochemical methods were used to detect the active fragments of caspase-3 and the expression of Bcl-2, Bcl-xl, Bax, iNOS, and nNOS. Results: Pretreatment with ginsenoside Rg1 (5.0 and 10.0 mg/kg) can make the substantia nigra dense band Nissl The loss of positive neurons and TH positive neurons was reduced, while the positive rate of TUNEL was decreased, accompanied by increased expression of Bcl-2 and Bcl-xl, decreased expression of Bax and iNOS, and inhibition of caspase-3 activation. Conclusion: Ginsenosides Rg1 pretreatment has a significant protective effect on MPTP-induced apoptosis of mouse substantia nigra neurons. Its effect may be through the reduction of iNOS and Bax protein expression and increase of Bcl-2 and Bcl-xl protein expression. Inhibition of caspase-3 activation achieved.