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目的:分离纯化半边旗中抗肿瘤活性成分6F(即7β,9-二羟基-15-氧-对映-贝壳杉烷-16-烯-19,6β-内酯)。方法:采用二氧化碳超临界萃取、结晶与重结晶、硅胶柱层析方法进行分离、纯化6F;采用HPLC-MS、HPLC和TLC检测6F的纯度。结果:经HPLC和TLC检测,可以得到6F的纯化合物。结论:采用结晶与重结晶、硅胶柱层析方法,可有效纯化半边旗中抗肿瘤成分6F。
OBJECTIVE: To isolate and purify the anti-tumor active ingredient 6F (ie, 7β, 9-dihydroxy-15-oxy-enantio-kaurene-16-en-19,6β-lactone) Methods: The supernatant was extracted by CO2, crystallized and recrystallized, and purified by silica gel column chromatography. The purity of 6F was determined by HPLC-MS, HPLC and TLC. Results: The pure compounds of 6F can be obtained by HPLC and TLC. Conclusion: Crystallization and recrystallization, silica gel column chromatography method can effectively purify the anti-tumor component 6F from Pteris semipinnata.