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1980-1982年在田间观察了大豆病毒病的症状共有八种,对其中表现为重花叶类型的毒株,经鉴别寄主反应、传染途径、抗性测定、电镜观察和血清学反应鉴定,结果如下:该病毒寄主范围较窄,在测定的19种植物中,除侵染大豆外,在菜豆接种叶片上表现浅黄色斑驳,回接大豆后呈系统花叶。此病毒在感病品种上很容易摩擦接种,发病率在40-83.33％;病株种子传病率高达68.42％,褐斑种子传病率高于无褐斑种子。种胚、子叶和种皮均能传病,以子叶传病率最高。测定12种蚜虫均能传病,其中以桃蚜、刺槐蚜传病率最高,杨蚜次之,该病毒的致死温度为65-70℃,稀释限点1:1000-6000,体外保毒期4-5天。用浸出法和2％磷钨酸负染,电子显微镜观察病毒粒体呈线条状,大小为760×13nm。用琼脂双扩散法测定病株汁液的血清反应呈阳性,在抗血清孔六和抗原孔穴之间形成白色沉淀线,呈直线。根据以上性状,鉴定河南大豆重花叶类型病毒病的毒源为大豆花叶病毒(SMV),该病为大豆花叶病。
In 1980-1982, eight symptoms of soybean virus disease were observed in the field. Among them, the strains characterized by heavy mosaic type were identified by host reaction, transmission route, resistance assay, electron microscope observation and serological reaction identification. Results As follows: The virus host narrower range, in the determination of the 19 kinds of plants, in addition to infecting soybean, inoculated leaves showed yellowish mottled mottled back to the soybean system was mosaic. The virus susceptible strains susceptible to friction vaccination, the incidence rate of 40-83.33%; diseased seed prevalence rate as high as 68.42%, brown spot seed transmission rate is higher than no brown spot seed. Seed embryo, cotyledon and seed coat can be disease, the highest incidence of cotyledons. Twelve kinds of aphids were tested for pathogenicity, of which Myzus persicae and Robinia pseudoacacia were the most prevalent, followed by Myzus persicae. The lethal temperature was 65-70 ℃, dilution limit 1: 1000-6000, 4-5 days. Levitation method and 2% phosphotungstic acid negative staining, electron microscopy virus particles were linear, the size of 760 × 13nm. Agar double diffusion method was used to determine the serum of the diseased plant was positive, forming a white sediment line between the anti-serum hole and antigen pore, showing a straight line. According to the above traits, the source of identification of heavy soybean mosaic virus disease in Henan is soybean mosaic virus (SMV), which is soybean leaf mosaic disease.