1989年1月南宁市某医院新生儿病室、婴儿室相继发生腹泻暴发,经病原学检查证实为鼠伤寒、山夫登堡沙门氏菌引起的医院内新生儿感染,现报道如下。材料与方法一、调查方法:查阅流行期间该院新生儿病历,对其发病情况及有关特征作调查。二、病原分离与鉴定:患者粪便、血及健康儿、产妇、医务人员粪便与外环境标本均按常规法进行细菌培养、鉴定。三、药敏试验:用Kirby-Bauer纸片扩散法。纸片购自中国药品生物制品检定所。四、质粒图谱与噬菌体分型:质粒提取 January 1989 Nanning City, a hospital neonatal wards, infants have been outbreaks of diarrhea, confirmed by pathological examination for typhoid fever, Salmonella Shaftesbury hospital caused by neonatal infection, are reported below. Materials and methods First, the survey method: access to the hospital during the epidemic neonatal medical records, the incidence and related characteristics of the investigation. Second, the pathogen isolation and identification: the patient’s stool, blood and healthy children, maternal, medical personnel excrement and external environment specimens were routinely bacterial culture, identification. Third, susceptibility testing: Kirby-Bauer paper diffusion method. Paper purchased from the China Pharmaceutical and Biological Products Inspection. Fourth, plasmid map and phage typing: plasmid extraction