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目的 探讨失血性休克大鼠L 选择素转录及翻译水平表达对创伤及休克病理生理过程的影响。方法 大鼠剪尾取血作为正常对照 ,颈部去皮颈动脉结扎剪断插管作为实验对照 ,建立失血性休克动物模型 ;遂将其分为正常对照组、实验对照组 (创伤组 )和实验休克组 (失血性休克组 ) ,每组均为 8只。用单克隆抗体标记 ,流式细胞仪检测大鼠失血休克急性期中性粒细胞表面L 选择素的动态表达 ,以及用半定量逆转录聚合酶链反应 (RT PCR)检测大鼠白细胞胞质L 选择素mRNA半定量变化。结果 正常对照组大鼠中性粒细胞表面不同时间点L 选择素表达量 (平均荧光道数 )之间的差异无显著性 (P >0 .0 5)。与正常对照组相比 ,实验对照组细胞表面及胞质mRNA表达均上调 ,细胞表面 3h达峰值 ,4、5h持续高水平 ;mRNA进行性升高 ,于 5h达峰值。与正常对照组比 ,实验休克组两者亦均上调 ;但与创伤组比 ,3h后则呈下调趋势。结论 创伤后中性粒细胞L 选择素表达上调 ,增强白细胞 内皮细胞黏附反应 ,有助于局部创伤愈合 ,修复并抵抗感染。休克组L 选择素表达下调 ,可减少白细胞 内皮细胞过度黏附及嵌塞毛细血管 ,有助于机体疏通微循环 ,改善灌流量
Objective To investigate the effects of L-selectin transcription and translation on the pathophysiology of hemorrhagic shock rats. Methods Tail blood from rats was used as normal control and neck peeled carotid artery ligation and cannula was taken as experimental control to establish hemorrhagic shock animal model. Then it was divided into normal control group, experimental control group (trauma group) and experiment Shock group (hemorrhagic shock group), each group was 8. Flow cytometry was used to detect the dynamic expression of L-selectin on the surface of neutrophils in the acute phase of hemorrhagic shock in rats, and the cytosolic L-selectin was detected by semi-quantitative reverse transcriptase-polymerase chain reaction (RT PCR) Semi-quantitative changes in mRNA. Results There was no significant difference in the expression of L-selectin (mean fluorescence channel number) at different time points on the neutrophil surface in normal control rats (P> 0.05). Compared with the normal control group, the cell surface and cytoplasmic mRNA expression were up-regulated in the experimental control group. The cell surface peaked at 3h and continued at a high level for 4,5h. The mRNA increased progressively and peaked at 5h. Compared with the normal control group, both experimental shock group were also increased; but compared with the trauma group, after 3h showed a downward trend. Conclusion The expression of L-selectin in traumatic neutrophils is up-regulated, which enhances the adhesion of leucocytes to endothelial cells and helps to heal local wounds and repair and resist infection. Shock group L-selectin expression down regulation, can reduce leukocyte endothelial cell adhesion and capillary occlusion, help the body to clear the microcirculation and improve perfusion