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本文采用人羊膜基膜(HABM)做为腹腔巨噬细胞、CCLM24黑色素瘤细胞和神经细胞生长的支持物,MTT定量法测定这三种细胞在HABM上的生长。发现CCLM 24黑色素瘤细胞在HABM上生长最为活跃,神经细胞次之,而巨噬细胞生长受到抑制。HABM经肝素酶Ⅰ、Ⅱ消化后,CCLM 24细胞生长更加活跃。胶原酶和软骨素酶ABC消化后其生长活性降低25％和30％。神经细胞在肝素晦Ⅰ消化的HABM上生长活跃,而其余酶消化的HABM均使神经细胞生长活性降低。提示不同的细胞在HABM上的生长性质不同。
In this study, human amniotic membrane-based membrane (HABM) was used as a supporter of peritoneal macrophages and CCLM24 melanoma cells and nerve cell growth. The growth of these three cells on HABM was determined by MTT assay. CCLM 24 melanoma cells were found to be most active on HABM, followed by nerve cells, while macrophage growth was inhibited. After HABM was digested with heparanase Ⅰ, Ⅱ, CCLM 24 cells grew more active. Collagenase and chondroitinase ABC decreased their growth activity by 25% and 30%. Nerve cells grew actively on HABM digested with Heparin II, whereas HABM digested by the remaining enzymes reduced neuronal growth activity. Suggesting that different cells have different growth properties on HABM.