论文部分内容阅读
目的 :观察 IL - 1β对基础状态及精氨酸加压素 (AVP)诱导心肌成纤维细胞 (CFs)增殖和 p2 7蛋白表达的影响。方法 :采用胰酶消化 ,差速贴壁法培养 CFs,四氮唑盐 (MTT)比色法检测细胞增殖 ,碘化丙啶 (PI)标记细胞DNA,间接免疫组化染色标记细胞内的 p2 7蛋白 ,用流式细胞分析仪 (FCM)技术测定细胞周期和 p2 7蛋白表达的阳性率。结果 :11× 10 - 7mol/ L AVP组 MTT法测定的 CFs的吸收 (A)值 (0 .386± 0 .0 11)较基础状态 (0 .32 4± 0 .0 0 7)明显升高 (P<0 .0 1) ;1× 10 5 U/ L IL - 1β单独作用组 (0 .2 98± 0 .0 30 )和 AVP+IL - 1β组 (0 .332± 0 .0 41)的 A值均分别较基础状态和 AVP组显著降低 (均 P<0 .0 1) ;2 IL- 1β组和 AVP+IL- 1β组 CFs的 S期细胞百分率和细胞增殖指数 (PI)分别较基础状态组和 AVP组明显降低 ,而 G0 / G1 期细胞百分率则分别高于上述两组 (均 P<0 .0 1) ;3IL- 1β组和 AVP+IL- 1β组 CFs的 p2 7蛋白表达阳性率分别为 (95 .0 3± 1.0 2 ) % ,(88.2 3± 2 .87) % ,分别高于基础状态 (78.45± 1.91) %和 AVP组 (6 3.30± 1.85 ) % ,并且有统计学意义 (均 P<0 .0 1)。结论 :IL - 1β通过升高细胞内 p2 7蛋白表达水平参与 CFs增殖的负调控过程。
AIM: To observe the effects of IL - 1β on basal state and arginine vasopressin (AVP) - induced proliferation of cardiac fibroblasts (CFs) and expression of p27 protein. Methods: CFs were cultured with trypsin digestion and differential adherent method. Cell proliferation was detected by MTT colorimetric assay, DNA was labeled with propidium iodide (PI) and p2 7 protein, the positive rate of cell cycle and p27 protein expression was determined by flow cytometry (FCM) technique. Results: The absorbance (A) value of CFs (0.386 ± 0.011) in the 11 × 10-7 mol / L AVP group was significantly higher than that in the basal state (0.324 ± 0.07) (P <0.01); 1 × 10 5 U / L IL - 1β alone group (0.228 ± 0.30) and AVP + IL - 1β group (0.332 ± 0.041) (P <0.01). The percentage of S phase cells and the cell proliferation index (PI) of CFs in 2 IL-1β group and AVP + IL-1β group were significantly higher than those in AVP group Basal state group and AVP group were significantly decreased, while the percentage of G0 / G1 phase cells were higher than those of the above two groups (all P <0.01). The p27 expression of CFs in 3IL-1β group and AVP + IL-1β group (95.3 ± 1.02)% and (88.2 3 ± 2.87)%, respectively, which were higher than those in the basal state (78.45 ± 1.91)% and AVP group (6 3.30 ± 1.85)%, respectively Significance (both P <0. 01). Conclusion: IL - 1β is involved in the negative regulation of CFs proliferation by increasing the level of p27 protein in cells.