论文部分内容阅读
目的比较酶联免疫吸附试验(ELISA)和逆转录巢式聚合酶链反应(RT-nPCR)检测HIV感染者血清中抗-HCV与HCVRNA的结果是否有差异。方法采用ELISA法和RTn-PCR法对300例HIV感染者的血清进行抗-HCV与HCVRNA检测。结果在300份血清标本中,抗-HCV与HCVRNA均为阳性者114份,占38%(114/300);抗-HCV阴性、HCVRNA阳性者41份,占13.67%(41/300);抗-HCV阳性、HCVRNA阴性者32份,占10.67%(32/300);两者均阴性113份,占37.67%(113/300);两种方法检测结果的一致率为75.67%,两种方法结果的差异无统计学意义(χ2=1.11,P﹥0.05)。结论 ELISA法与RTn-PCR法在HIV感染者中诊断丙型肝炎其结果差异无统计学意义,但二者联合检测可提高检出率,在HIV感染者中存在免疫抑制造成血清学结果假阴性率较高,因此,对HIV感染者中抗-HCV阴性者应进行HCVRNA检测,降低漏诊率。
Objective To compare the results of anti-HCV and HCV RNA in serum of HIV-infected patients by ELISA and RT-nPCR. Methods Anti-HCV and HCV RNA were detected in sera of 300 HIV-infected persons by ELISA and RT-PCR. Results Among the 300 serum samples, 114 were positive for anti-HCV and HCV RNA, accounting for 38% (114/300), 41 were HCV-negative and HCVRNA-positive, accounting for 13.67% (41/300) -HCV positive, 32 HCVRNA-negative, accounting for 10.67% (32/300); 113 negative, both of which were 37.67% (113/300); the consistency of the two methods was 75.67% The difference was not statistically significant (χ2 = 1.11, P> 0.05). Conclusion The results of ELISA and RTn-PCR in the diagnosis of hepatitis C in HIV-infected patients showed no significant difference. However, the combination of ELISA and RTn-PCR could increase the detection rate, and the immunosuppression in HIV-infected patients had false-negative serological results Therefore, HIV-negative HCV-infected individuals should be tested for HCV RNA to reduce the rate of misdiagnosis.