论文部分内容阅读
目的探讨TFF3、survivin、CD147、CD34在不同胃黏膜的表达及其相关性。方法利用组织芯片技术构建包括30例正常胃黏膜和272例患者(浅表性胃炎30例、萎缩性胃炎30例、不典型增生30例及胃癌182例)胃黏膜的组织芯片,同时采用SP免疫组化方法检测TFF3、survivin、CD147、CD34表达。结果萎缩性胃炎、不典型增生和胃癌胃黏膜TFF3表达高于浅表性胃炎和正常对照(P<0.01);胃癌胃黏膜survivin、CD147和CD34表达高于正常胃黏膜、浅表性胃炎、萎缩性胃炎和不典型增生(P<0.05),不典型增生胃黏膜survivin表达高于萎缩性胃炎(P<0.05),浅表性胃炎胃黏膜CD147和CD34表达高于正常胃黏膜(P<0.05)。TFF3表达与胃癌组织类型、淋巴结转移和TNM分期有关(P<0.05),survivin、CD147和CD34表达与胃癌浸润深度、淋巴结转移和TNM分期有关(P<0.05)、CD147表达与胃癌分化程度有关(P<0.01)。TFF3、survivin、CD147阳性表达的MVD高于阴性(P<0.01)。结论TFF3、CD147和CD34可能参与胃黏膜保护及修复,在胃黏膜癌变过程中,TFF3、survivin、CD147、CD34表达逐渐上调,对胃癌发生发展、浸润转移和血管形成有促进作用,可作为早期诊断胃癌和预测其发生转移的重要指标。
Objective To investigate the expression of TFF3, survivin, CD147 and CD34 in different gastric mucosa and their correlation. Methods Tissue microarray was used to construct tissue microarray including 30 normal gastric mucosa and 272 gastric mucosa in 272 patients (30 cases of superficial gastritis, 30 cases of atrophic gastritis, 30 cases of atypical hyperplasia and 182 cases of gastric cancer) The histochemical method was used to detect the expression of TFF3, survivin, CD147 and CD34. Results The expression of TFF3 in gastric mucosa of atrophic gastritis, atypical hyperplasia and gastric cancer was higher than those in superficial gastritis and normal controls (P <0.01). The expressions of survivin, CD147 and CD34 in gastric mucosa were significantly higher than those in normal gastric mucosa, superficial gastritis and atrophy (P <0.05). The expression of survivin in atypical hyperplastic gastric mucosa was higher than that in atrophic gastritis (P <0.05), while the expression of CD147 and CD34 in superficial gastritis was higher than that in normal gastric mucosa (P <0.05) . The expressions of survivin, CD147 and CD34 were correlated with the depth of invasion, lymph node metastasis and TNM staging (P <0.05). The expression of CD147 correlated with the degree of differentiation of gastric cancer (P <0.05). The expression of TFF3 was correlated with the histological type, lymph node metastasis and TNM stage P <0.01). MVD of TFF3, survivin and CD147 were higher than negative (P <0.01). Conclusions TFF3, CD147 and CD34 may be involved in the protection and repair of gastric mucosa. The expression of TFF3, survivin, CD147 and CD34 may be up-regulated during the carcinogenesis of gastric mucosa. TFF3, CD147 and CD34 may promote the development, infiltration, metastasis and angiogenesis of gastric carcinoma. Gastric cancer and predict their occurrence of an important indicator of metastasis.