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目的观察1,25-(O H)2D对阿霉素肾病大鼠血管紧张素转化酶(AC E)表达的影响。方法1283只周龄相同体重相近的雄性Sprague D aw ley(SD)大鼠随机分为4组;正常对照组、肾病组、激素治疗组和激素+阿法D治疗组(简称联合组)。尾静脉注射阿霉素制备大鼠阿霉素肾病模型,实验当天、第14天、第283天及第42天每组随机抽取8只大鼠取血、尿与肾脏,测血中BU N、C r、A lb、C H O、C a、P、A LP的浓度,24h尿蛋白排泄量血浆与肾皮质AC E浓度,病理积分评价大鼠肾脏损害程度。结果实验初,各组大鼠血中BU N、C r、A lb、C H O、C a、P、A LP的浓度、24h尿蛋白排泄无明显差异,自实验第14天起,肾病组、激素组与联合组大鼠血BU N、C r、C H O、A LP含量、24h尿蛋白排泄量较正常组增高,A lb、C a、P含量较正常组降低(P<0.01)。自实验14天起,激素组与联合组C a、P浓度高于肾病组(P<0.01),而联合组高于激素组(P<0.01)。血中A C E浓度无明显变化,肾局部AC E浓度经激素治疗后明显受抑,但联合治疗效果更显著(P<0.01)。实验末,肾脏病理积分依次为肾病组、激素组、联合组、正常组。相关分析发现:血A lb、C a、P浓度与病理积分呈负相关(P均<0.01),肾皮质AC E、BU N、C r浓度与病理积分呈正相关(P<0.01),血C a、P浓度与肾皮质A C E浓度呈?
Objective To investigate the effect of 1,25- (O H) 2D on angiotensin-converting enzyme (ACE) expression in adriamycin-induced nephropathy rats. Methods 1283 male Sprague Dawley (SD) rats of the same body weight and the same age were randomly divided into 4 groups. The normal control group, nephrosis group, hormone therapy group and hormone + Alfa D treatment group (abbreviated as combined group). Adriamycin was injected into the caudal vein to prepare rat adriamycin nephropathy model. On the day of experiment, blood, urine and kidneys of 8 rats were randomly selected from the 14th day, the 283th day and the 42nd day. BU N, The concentrations of C, A, B, CHO, C a, P and A LP, the urinary protein excretion in 24 h, the AC E concentration in plasma and renal cortex, and the pathological score were used to evaluate the degree of renal damage in rats. Results At the beginning of the experiment, there was no significant difference in the concentrations of BU N, C r, A lb, CHO, C a, P and A LP and the urinary excretion of urine in all groups. From the 14th day of experiment, The levels of BU N, C r, CHO, A LP and 24h urinary protein excretion were higher than those in normal group (P <0.01). From the 14th day of experiment, the concentrations of C a and P in hormone group and combination group were higher than those in nephropathy group (P <0.01), but higher in combined group than in hormone group (P <0.01). Blood A C E concentration had no significant change in the local renal AC E concentration by hormone therapy was significantly inhibited, but the combined treatment effect was more significant (P <0.01). At the end of experiment, the pathological integrals of kidney were nephropathy, hormone group, combination group and normal group. Correlation analysis showed that there was a negative correlation between the concentrations of blood lb, C a, P and the pathological scores (all P <0.01), and the levels of AC E, BU N and C r were positively correlated with the pathological scores (P <0.01) a, P concentration and renal cortex ACE concentration was?