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目的 :探讨神经损伤诱导蛋白2(NINJ2)基因多态性与陕西地区缺血性脑卒中的关系。方法 :选取2014年1月~2016年2月在我院治疗的缺血性脑卒中患者172例作为观察组,同时选取在我院进行体检的110例健康人作为对照组,采用聚合酶联反应-限制性片段长度多态性技术对NINJ2 rs10849373、rs4980840、rs2535393和rs11063806位点进行基因型检测,比较观察组和对照组基因型和等位基因频率的差异。结果 :观察组体重指数(BMI)、血清总胆固醇(TC)和低密度脂蛋白(LDL)分别为(25.22±1.89)kg/m~2、(4.81±1.22)mmol/L和(3.01±0.82)mmol/L,吸烟和饮酒的比例分别为40.70%和27.91%,均高于对照组,差异比较有统计学意义;rs10849373、rs4980840、rs2535393和rs11063806位点GG、GA和AA基因型在观察组和对照组的频率分布符合Hardy-Weinberg平衡定律;观察组rs10849373位点AA、GA基因型以及等位基因A的频率分别为11.63%、40.12%和31.69%,明显高于对照组,rs10849373位点的基因型及基因频率在观察组和对照组间差异有统计学意义;rs4980840、rs2535393和rs11063806位点在观察组和对照组间的分布差异无统计学意义。结论 :NINJ2基因中rs10849373位点与陕西地区缺血性脑卒中有关,需进一步研究。
Objective: To investigate the relationship between NINJ2 gene polymorphism and ischemic stroke in Shaanxi Province. Methods: A total of 172 patients with ischemic stroke who were treated in our hospital from January 2014 to February 2016 were selected as the observation group, and 110 healthy subjects in our hospital were selected as the control group. Polymerase chain reaction The restriction fragment length polymorphism (RFLP) technique was used to detect the genotype of NINJ2 rs10849373, rs4980840, rs2535393 and rs11063806 loci. The difference of genotype and allele frequencies between the observation group and the control group was compared. RESULTS: The body mass index (BMI), serum total cholesterol (TC) and low density lipoprotein (LDL) in the observation group were (25.22 ± 1.89) kg / m 2, (4.81 ± 1.22) mmol / L and ) mmol / L, smoking and drinking were 40.70% and 27.91%, respectively, which were higher than the control group, the difference was statistically significant; rs10849373, rs4980840, rs2535393 and rs11063806 GG, GA and AA genotypes in the observation group The frequencies of AA and GA genotypes and allele A of rs10849373 in observation group were 11.63%, 40.12% and 31.69%, respectively, which were significantly higher than those in control group and rs10849373 The genotypes and frequencies of the two groups were statistically different between the observation group and the control group. There was no significant difference in the distribution of rs4980840, rs2535393 and rs11063806 between observation group and control group. Conclusion: The rs10849373 locus in NINJ2 gene is associated with ischemic stroke in Shaanxi Province, which needs further study.